NATIONAL STANDARD METHOD
INVESTIGATION OF CEREBROSPINAL FLUID
BSOP 27
Issued by Standards Unit, Evaluations and Standards Laboratory Centre for Infections
Fulltext

INDEX
STATUS OF NATIONAL STANDARD METHODS................................................................................ 2
INDEX...................................................................................................................................................... 3
AMENDMENT PROCEDURE................................................................................................................. 4
SCOPE OF DOCUMENT ........................................................................................................................ 5
INTRODUCTION ..................................................................................................................................... 5
TECHNICAL INFORMATION/LIMITATIONS ....................................................................................... 10
1 SAFETY CONSIDERATIONS ....................................................................................................... 11
1.1 SPECIMEN COLLECTION ............................................................................................................. 11
1.2 SPECIMEN TRANSPORT AND STORAGE ........................................................................................ 11
1.3 SPECIMEN PROCESSING............................................................................................................. 11
2 SPECIMEN COLLECTION ............................................................................................................ 12
2.1 OPTIMAL TIME OF COLLECTION ................................................................................................... 12
2.2 CORRECT SPECIMEN TYPE AND METHOD OF COLLECTION ............................................................. 12
2.3 ADEQUATE QUANTITY AND APPROPRIATE NUMBER OF SPECIMENS ................................................ 12
3 SPECIMEN TRANSPORT AND STORAGE ................................................................................. 12
3.1 TIME BETWEEN SPECIMEN COLLECTION AND PROCESSING............................................................ 12
3.2 SPECIAL CONSIDERATIONS TO MINIMISE DETERIORATION ............................................................. 12
4 SPECIMEN PROCESSING ........................................................................................................... 12
4.1 TEST SELECTION ....................................................................................................................... 12
4.2 APPEARANCE ............................................................................................................................ 13
4.3 MICROSCOPY............................................................................................................................ 13
4.4 CULTURE AND INVESTIGATION .................................................................................................... 15
4.5 IDENTIFICATION ......................................................................................................................... 16
4.6 ANTIMICROBIAL SUSCEPTIBILITY TESTING.................................................................................... 17
5 REPORTING PROCEDURE.......................................................................................................... 17
5.1 APPEARANCE ............................................................................................................................ 17
5.2 MICROSCOPY............................................................................................................................ 17
5.3 CULTURE .................................................................................................................................. 18
5.4 ANTIMICROBIAL SUSCEPTIBILITY TESTING ................................................................................... 18
6 REPORTING TO THE HPA (LOCAL AND REGIONAL SERVICES AND CENTRE FOR
INFECTIONS)........................................................................................................................................ 19
7 ACKNOWLEDGEMENTS AND CONTACTS................................................................................ 20
APPENDIX ............................................................................................................................................ 21
REFERENCES ...................................................................................................................................... 22

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